Molecular-genetic characterization of thylakoid protein phosphorylation in Arabidopsis thaliana [Elektronische Ressource] / vorgelegt von Vera Bonardi

Molecular-genetic characterization of thylakoid protein phosphorylation in Arabidopsis thaliana Dissertation zur Erlangung des Doktorgrades der Fakultät für Biologie der Ludwig-Maximilians-Universität München Vorgelegt von Vera Bonardi Aus Iseo, Brescia Italien München 02.08.2006 Berichterstatter: Prof. Dr. Dario Leister Prof. Dr. Jürgen Soll Tag der mündlichen Prüfung: 25.09.2006 TABLE OF CONTENTS ABBREVIATIONS 1 SUMMARY 3 ZUSAMMENFASSUNG 5 1. INTRODUCTION 7 1.1 STATE TRANSITIONS 8 1.2 ACCLIMATION BY ADJUSTMENT OF PHOTOSYSTEM STOICHIOMETRY 13 1.3 TURNOVER OF PSII UNITS 15 2. MATERIALS AND METHODS 19 2.1 DATABASE ANALYSIS, DIGITAL NORTHERN, AND PREDICTION OF SUBCELLULAR TARGETING 2.2 PLANT LINES AND PROPAGATION 20 2.3 NUCLEIC ACID ANALYSIS 2.4 PIGMENT ANALYSIS 21 2.5 IMMUNOBLOT ANALYSIS 22 2.6 In-vitro PROTEIN IMPORT IN PURIFIED CHLOROPLASTS 22 2.7 INTRACELLULAR LOCALIZATION OF dsRED FUSIONS 23 2.8 CHLOROPHYLL FLUORESCENCE MEASUREMENTS 24 2.9 LHCII PHOSPHORYLATION ANALYSIS 25 2.10 IMMUNOBLOT ANALYSIS OF D1 PROTEIN UPON EXPOSURE TO HIGH-INTENSITY LIGHT 26 2.11 PULSE-CHASE MEASUREMENT OF D1 TURNOVER 26 2.12 MEASUREMENT OF ACCLIMATION TO CHANGES IN LIGHT QUALITY 27 2.13 mRNA EXPRESSION PROFILING 28 2.14 2D BN/SDS-PAGE ANALYSIS OF THYLAKOID PROTEINS 28 3. RESULTS 30 3.