Molecular background of oxygen sensitivity in [FeFe] hydrogenases [Elektronische Ressource] / submitted by Sven T. Stripp

Molecular Background of Oxygen Sensitivity in [FeFe] hydrogenases Dissertation to obtain the degree Doctor Rerum Naturalium (Dr. rer. Nat.) at the faculty Biology and Biotechnology Ruhr-University Bochum International Graduate School of Biosciences Ruhr-University Bochum Department of Plant Biochemistry Photobiotechnology submitted by Sven T. Stripp Bochum February 2010 I Molekulare Grundlagen der Sauerstoffsensitivität von [FeFe]-Hydrogenasen Dissertation zur Erlangung des Grades eines Doktors der Naturwissenschaften der Fakultät für Biologie und Biotechnologie an der internationalen Graduiertenschule Biowissenschaften der Ruhr-Universität Bochum angefertigt im Lehrstuhl Biochemie der Pflanzen AG Photobiotechnologie vorgelegt von Sven T. Stripp aus Wesel Bochum Februar 2010 II Meinen geliebten Füchsen What is happening to my skin? Where is that protection that I needed? Air can hurt you too Air can hurt you too Some people say not to worry about the air Some people never had experience with... Air... Talking Heads III TABLE OF CONTENTS Table of figures Introduction 1.1 Hydrogenases catalyse uptake and release of dihydrogen 1 Hydrogen in enlivened Nature 1 Convergent evolution gave three classes of hydrogenases 2 Structural differentiation in [FeFe] hydrogenases 5 The minimal [FeFe] hydrogenases in green algae 6 The C.