Molecular analysis of factors involved in biogenesis of the cyanobacterial thylakoid membrane [Elektronische Ressource] / vorgelegt von Marco Schottkowski

Molecular analysis of factors involved in biogenesis of the cyanobacterial thylakoid membrane Dissertation zur Erlangung des Grades eines Doktors der Naturwissenschaften an der Fakultät für Biologie der Ludwig-Maximilians Universität München vorgelegt von Marco Schottkowski München, Dezember 2009 Referent: Prof. Dr. Jörg Nickelsen, AG Molekulare Pflanzenwissenschaften Korreferent: Prof. Dr. Jürgen Soll, AG Biochemie und Physiologie der Pflanzen Tag der Abgabe: 15. Dezember 2009 TABLE OF CONTENTS SUMARY 1 ZSAMENFASUNG 3 INTRODUCTION 5 Photosynthesis 5 Photsytem I 7 Photosystem II reaction center protein D1 9 Chlorophyll synthesis 11 Biogenesis of thylakoid membranes 12 Asembly factors forPSI 12 Assembly factors for PSII 14 Spatial organization of thylakoid biogenesis in cyanobacteria 16 TPR proteins and photosynthesis 19 Synechocystis sp. PCC6803 as a model organism 21 Aims ofthis work 23 RESULTS 24 Section I: Interaction of the periplasmic PratA factor and the PsbA (D1) protein during biogenesis of photosystem II in Synechocystis sp. PCC6803 27 Section II: Pitt, a novel tetratricopeptide repeat protein involved in light dependent chlorophyll biosynthesis and thylakoid membrane biogenesis in Synechocystis sp.