Molecular analyses of the pathogenic interaction formed between the model legume Medicago truncatula and the oomycete Aphanomyces euteiches [Elektronische Ressource] / von Oyunbileg Nyamsuren

Molecular Analyses of the PathogenicInteraction Formed Between the Model LegumeMedicago truncatula and the OomyceteAphanomyces euteichesDem Fachbereich Biologie der Universität Hannoverzur Erlangung des GradesDoktorin der NaturwissenschaftenDr. rer. nat.genehmigte DissertationvonOyunbileg Nyamsurengeboren am 08.03.1976in Süd-Gobi aimag, Mongolei2004Referent : Prof. Dr. Hans-Jörg JacobsenKorreferent : PD Dr. Philipp FrankenTag der Promotion : 29. Januar 2004Dedicated to my mother Odsuren Darjaa.Χайрт ээждээ з ориул ав.iABSTRACTCommon root rot of pea, which is the major yield – reducing factor for peaproduction world-wide is caused by the oomycete Aphanomyces euteiches. Themodel legume Medicago truncatula was chosen to study the molecular mechanismsof this disease. Young seedling of M. truncatula were effectively infected withzoospores of A. euteiches and similar disease development as in pea was observed. A.euteiches colonizes the root system and the disease symptoms occur mainly in theplant root. Therefore the root tissue was used for the analyses. cDNA-AFLP methodwas used to determine the time point of disease development where the mosttranscriptional changes occur. The earliest time point determined, was chosen toestablish a SSH-cDNA-library enriched for the genes preferentially expressed inplant root after infection with oomycete. In total 560 ESTs from this library weresequenced and annotated.