Modulation of growth factor function by additional extracellular signals in CNS neurones and glia [Elektronische Ressource] / Nadhim Bayatti

Abteilung Anatomie und ZellbiologieUniversität UlmLeiter: Prof. Dr. Dr. h.c. Ch. PilgrimModulation of growth factor function by additional extracellular signalsin CNS neurones and gliaDissertationzur Erlangung des Doktorgrades der Humanbiologiean der Medizinische Fakultätder Universität Ulmvorgelegt von Nadhim Bayatti MSc., ARCSaus Bagdad, Irak2001Amtierender Dekan: Prof. Dr. med. P. Gierschik1. Gutachter: H.D. Dr. J. Engele2. Gutachter: P.D. Dr. J. WaltenbergerTag der Promotion: 14.12.2001In memory of my father, Noman N. BayattiContentsAbbreviations 11 Introduction 41.1 Fibroblast growth factors (FGFs) 41.2 FGF receptors 51.3 FGF signalling- the FGFR as a typical tyrosine kinase receptor 71.4 Activities of FGFs during neural development 81.5 EGF family 101.6 EGFR signalling 111.7 TGFα during neural development 121.8 Neurotrophins 131.9 Neurotrophin signalling 141.10 Actions of neurotrophins during development 141.11 Concerted effects of epigenetic factors 151.12 Aims of the study 162 Materials and Methods 182.1 Reagents 182.2 Antibodies 202.3 PCR primers 212.4 Solutions 222.5 Materials 272.6 Equipment 272.7 Animals 282.8 Cell culture 282.8.1 Treatment of cell cultures 292.9 Immunocytochemistry 302.10 Fos expression assay 302.11 Glial proliferation assay 302.12 Cell survival assay 312.13 RNA isolation 312.14 RT-PCR 312.15 Cell fractionation 322.16 Western blotting 322.17 EMSA 332.