Micropipetter Tutorial

Appendix B:Micropipetter TutorialHow Not to Break A Micropipetter!IntroductionTh ese machines are EXPENSIVE. Be sure to follow these Micropipetters are continuously adjustable pipettes which simple rules:are used to draw up and deliver specifi ed volumes. Th e 1. Do NOT dial a pipetter below 0volume is determined by adjusting the volume adjustment 2. Do NOT dial a pipetter above their maximum valueknob. Th e volume, in microliters, is displayed in the volume 3. Do NOT drop a pipetterindicator. Th e fi rst section describes the parts and general use 4. Do NOT submerge the shaft of a pipetter in liquidof a micropipetter. Th e second section describes how to use a 5. Do NOT pound the pipetter into a tip micropipetter and includes an exercise. Refer to the micropi-6. DO think about what you’re doing and DO use common petter diagram on the next page as you read this tutorial.sense!Metric conversions: small volumesB. Micopipetter InstructionsTh e two most prevalent units of liquid measurement in Your instructor will demonstrate the proper use of micropi-molecular biology are the milliliter (mL) and the microliter petters and sterile technique. Although the instructions below (μL).apply specifi cally to the P-20, they are also applicable to the P-200 and P-1000. Refer to Figure 1 on the next page.1 mL = 0.001 liter or 1/1,000 liter 1,000 mL = 1 liter1 μL = 0.000001 liter or 1/1,000,000 liter 1,000,000 μL = 1 liter1 μL = 0.001 mL or 1/1000 mL 1,000 ...