Med12 is an essential coordinator of gene regulation during mouse development [Elektronische Ressource] / by Pedro Pereira da Rocha

Med12 is an essential coordinator of gene regulation during mouse development Inaugural-Dissertation to obtain the academic degree Doctor rerum naturalium (Dr. rer. nat.) submitted to the Department of Biology, Chemistry and Pharmacy of Freie Universität Berlin by Pedro Pereira da Rocha from Vila Nova de Gaia, Portugal November 2010 The work here described was performed at the Max-Planck Institute for Molecular Genetics in Berlin between January 2007 and November 2010 under the supervision of Dr. Heinrich Schrewe. st1 Reviewer: Prof. Dr. Bernhard G. Herrmann Max-Planck Institute for Molecular Genetics Ihnestrasse 73 D-14195 Berlin nd2 Reviewer: Prof. Dr. Reinhard Kunze Institute of Biology - Applied Genetics Albrecht-Thaer-Weg 6 D-14195 Berlin Date of defence: 07/02/2011 Aos meus pais Table of Contents 1  An introduction to eukaryotic transcription and the Mediator 9 1.1  Coregulators of eukaryotic transcription 11 1.2  The Mediator complex 13 1.2.1  Mediator control of Pol II and the subunits required for all functions of the complex 15 1.2.2  The CDK8 module 18 1.2.3  Mediator subunits with gene-specific functions 19 1.3  The Mediator subunit MED12 22 1.3.1  MED12 and its general role in transcription 22 1.3.2  Gene-specific functions of MED12 23 1.3.3  Mutations in MED12 that cause human diseases 25 1.