Mass spectrometry based proteomics approaches unraveling dynamic processes in the entire bacterial cell [Elektronische Ressource] / Andreas Otto

    Mass spectrometry based proteomics approaches unraveling dynamic processes in the entire bacterial cell Inauguraldissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer.nat.) an der Mathematisch-Naturwissenschaftlichen Fakultät der Ernst- Moritz- Arndt- Universität Greifswald vorgelegt von Andreas Willy Otto geboren am 21.06.1979 in Kiel Greifswald, den 6.12.2010              Dekan: Prof. Dr. K. Fesser 1. Gutachter Prof. Dr. M. Hecker 2. Gutachter Prof. Dr. J. M. van Dijl Tag der Promotion: 11.02.2011    Content  ______________________________________________________________________________  Content Sumary 3 Zusamenfasung 5 Introduction 7 1. Protemics 7 1.1. 2D GE based proteomics 7 1.2. Mass spectrometry based proteomics 8 1.2.1. Technological and conceptual prerequisites for success of mass spectrometry in life sciences 9 2. Towards the comprehensive proteomic description of the gram positive model organism Bacillus subtilis 13 2.1. Cytosolic proteins 14 2.2. Extracellular 2.3. Membrane proteins 2.4. Surface 15 3. Fractionation techniques applied in mass spectrometry based proteomics 17 3.1. Chromatographic/electrophoretic fractionation methods 17 3.1.1. GeLCMS 17 3.1.2.