Malaria and host erythrocyte channels [Elektronische Ressource] / vorgelegt von Valérie Tanneur

Malaria and host erythrocyte channels Dissertation der Fakultät für Biologie der Eberhard Karls Universität Tübingen zur Erlangung des Grades eines Doktors der Naturwissenschaften vorgelegt von Valérie TANNEUR aus Aix-en-Provence (Frankreich) 2005 Tag der mündlichen Prüfung 12/10/04 Dekan : Prof. Dr. Schöffl 1. Berichterstatter : Prof. Dr. Florian Lang 2. Berichterstatter : Prof. Dr. Friedrich Götz CONTENTS CONTENTS_____________________________________________________________ 1 ABBREVIATIONS _______________________________________________________ 3 SUMMARY 5 ZUSAMMENFASSUNG __________________________________________________ 7 INTRODUCTION________________________________________________________ 9 1/ Malaria: general features and Plasmodium spp. life cycle.______________________ 9 2/ Membrane transport in the non-infected erythrocytes. ________________________ 13 3/ Plasmodium infection and erythrocyte membrane changes.____________________ 17 4/ Oxidative stress and activation of the NPPs in Plasmodium-infected erythrocytes. _ 26 5/ Purinoceptors in erythrocytes ___________________________________________ 28 6/ Channel-induced “apoptosis” of Plasmodium falciparum infected erythrocytes. ___ 32 OBJECTIVES OF THE STUDY 34 MATERIALS AND METHODS 35 1/ Preparation of human erythrocytes. ______________________________________ 35 2/ In vitro culture of P. falciparum infected human erythrocytes.