Light microscopic precise measurements of genome structure during tumorigenesis in transgenic mice [Elektronische Ressource] / presented by Wei Jiang

Dissertation submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto-Carola University of Heidelberg, Germany for the degree of Doctor of Natural Sciences presented by Wei Jiang, M.Sc. (Medical Physics) born in: Hebei, China Oral examination: 1 February 2010 Light Microscopic Precise Measurements of Genome Structure during Tumorigenesis in Transgenic Mice Referees: Prof. Dr. Dr. Christoph Cremer Prof. Dr. Stefan Wölfl AbstractThe higher order and spatial organisation of the genome is closely relatedto gene’s transcriptional activity. With the numerous results reported in thisarea, littleisknownaboutthepreciseinformationofspecificchromatinstruc-ture, especially the dynamic during physiological changes and tumorigenesis,in the well-preserved tissue sections. In this work, thin tissue cryosections(about 200 nm in thickness) from the mammary gland of transgenic micewereusedtostudythegenomeorganisationduringthetumorigenesisprocess.Stereological methods were used to estimate the three-dimensional genomestructure from the two-dimensional nuclear profiles. It was found that thewhole genome-wide chromatin condensation state varies significantly duringthe tumorigenesis process.