Leishmania tropica [Elektronische Ressource] : molecular epidemiology, diagnosis and development of an axenic amastigote model / von Abedelmajeed Naser Eddin

Aus dem Institut für Mikrobiologie und Hygiene der Medizinischen Fakultät Charité – Universitätsmedizin Berlin und demKuvin Center for the Study of Infectious and Tropical Diseases, The Hebrew University, Hadassah Medical School, Jerusalem, Israel DISSERTATION Leishmania tropica: Molecular Epidemiology, Diagnosis and Development of an Axenic Amastigote Model zur Erlangung des akademischen Grades Doctor rerum medicarum (Dr. rer. medic.) vorgelegt der Medizinischen Fakultät Charité – Universitätsmedizin Berlin von Abedelmajeed Naser Eddin aus Palästina 1 This research has been part of a German-Israeli-Palestinian cooperation project on the Emergence of Cutaneous Leishmaniasis in the Middle East: An investigation of Leishmania tropica in the Palestinian Authority and Israel. Granted by the Deutsche Forschungsgemeinschaft (Scho 448/8-1). Gutachter: 1. Prof. Dr. med. H.-W. Presber 2. Prof. Dr. A. Warburg 3. Prof. Dr. R. Ignatius thDatum der Promotion: 19 November 2010 2 Table of Contents List of abbreviations 4 1. Abstract 5 Zusammenfassung 6 2. Introduction 8 3. Materials and Methods 11 3.1 Development of new Leishmania tropica diagnostic method 3.11 Samples and Leishmania species reference strains 11 3.12 ITS1-PCR and RFLP 11 3.13 PCR followed with RLB 12 3.2 L.