Isothiocyanate metabolism in generalist lepidopteran herbivores [Elektronische Ressource] / Katharina Schramm. Gutachter: Jonathan Gershenzon ; Klaus-Jürgen Appenroth ; Raymond Barbehenn

Isothiocyanate metabolism in generalist lepidopteran herbivoresDissertationzur Erlangung des akademischen Grades doctor rerum naturalium(Dr. rer. nat.)vorgelegt dem Rat der Biologisch-Pharmazeutischen Fakultät der Friedrich-Schiller-Universität Jenavon Dipl.-BiologinKatharina Schrammgeboren am 7. August 1977 in BerlinJena, November 2010Gutachter: Prof. Dr. Jonathan Gershenzon; Max Planck Institut für Chemische Ökologie, JenaPD Dr. Klaus Appenroth; Universität Jena, JenaProf. Raymond Barbehenn, PhD; University of Michigan, Ann Arbor, USATag der öffentlichen Verteidigung: 14. Februar 2011 Research is the process of going up alleys to see if they are blind. Marston Bates Table of Contents1 General Introduction 1 The glucosinolate myrosinase system 2 Isothiocyanates and their metabolism in mammals and insects 5 The role of GSTs and glutathione in isothiocyanate metabolism in mammals 8 Role of GSTs and glutathione in isothiocyanate metabolism in generalist insect herbivores 9 2 Metabolism of isothiocyanates in generalist lepidopteran herbivores by conjugation with glutathione 11 2.1 Introduction 12 2.2 Materials and Methods 15 2.3 Results 19 2.3.1 The GSH conjugate of 4-methylsulfinylbutyl isothiocyanate (4msob-ITC) is excreted in Spodoptera littoralis faeces after feeding on A. thaliana Col-0 19 2.3.2 Isothiocyanates of varying structure are metabolized to GSH conjugates in S. littoralis 22 2.3.