Investigations on the rapid transbilayer movement of phospholipids in biogenic membranes [Elektronische Ressource] / von Janek Kubelt

Investigations on the rapid transbilayer movement of phospholipids in biogenic membranes Dissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) im Fach Biophysik eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät I der Humboldt-Universität zu Berlin von Diplom Biophysiker Janek Kubelt geborem am 07. März 1972 in Neustrelitz Präsident der Humboldt-Universität zu Berlin: Prof. Dr. Jürgen Mlynek Dekan der Mathematisch-Naturwissenschaftlichen Fakultät I: Prof. Dr. Michael Linscheid Gutachter: 1. Prof. Dr. A. Herrmann 2. Prof. Dr. T. Pomorski 3. Prof. Dr. A.K. Menon Tag der mündlichen Prüfung: 16.04.2004 Table of contents 1 1 Abbreviations 4 1 Introduction 6 1.1 Phospholipid transmembrane movement in eukaryotic cells 8 1.2 The architecture and dynamic of the bacterial envelope 11 1.2.1 The membrane organization of E.coli 11 1.2.2 Glycerophospholipids in E.coli 13 1.2.3 Phospholipid movement across bacterial membranes 14 1.3 Methods for the characterization of transmembrane distribution and movement of phospholipids in biological membranes 17 1.3.1 Assays for the determination of transmembrane movement and distribution of endogenous phospholipids 17 1.3.2 embrane movement and distribution of phospholipid analogues 18 2 Scope 22 3 Material and Methods 24 3.1 Chemicals 24 3.2 Preparation of inverted inner membrane vesicles from E.