Investigations on influence of different dietary proteins on lipid metabolism in rats fed a hyperlipidemic diet [Elektronische Ressource] / presented by Anjali Shukla

Institute of Agricultural and Nutritional Sciences (Director: Prof. Dr. M. Rodehutscord) of the Faculty of Natural sciences III (Dean: Prof. Dr. P. Wycisk) of the Martin-Luther-University Halle-Wittenberg, Germany “Investigations on influence of different dietary proteins on lipid metabolism in rats fed a hyperlipidemic diet” Dissertation to achieve the degree of Doctor of Nutritional Sciences (Dr. troph.) presented by Anjali Shukla (Master of Science) born on 1. Juli 1977 in Sitapur (India) Referee: Prof. Dr. Habil. Klaus Eder Prof. Dr. Habil. Gabriele I. Stangl Prof. Dr. Habil. Gerald Rimbach Defence on: 12.02.2007 Halle/Saale 2007 urn:nbn:de:gbv:3-000011438[http://nbn-resolving.de/urn/resolver.pl?urn=nbn%3Ade%3Agbv%3A3-000011438] C ONTENTS I. Contents 1. Introduction 1 2. Materials and Methods 7 2.1. Animal Studies 2.2. Characterisation of the Diet 7 2.3. Analysis of the Experimental Diets 10 2.4. Processing Dietary Proteins 12 2.5. Analysis of the 2.6. Sample Preparation 20 2.6.1. Blood, Plasma and Lipoproteins 20 2.6.2. Preparation of Homogenate, Cytosol and Microsomes 20 2.6.3. Extraction of total Lipids from the Liver and Faeces 21 2.7. Analytical Methods 21 2.7.1. Cholesterol and Triglyceride Determination 21 2.7.2. Estimation of Apolipoproteins 22 2.7.3. Amino Acids Analysis in Plasma 23 2.7.4.