Investigations on cellular nanoparticles required for synthesis of chitin the precursor for chitosan [Elektronische Ressource] / vorgelegt von Mayur Kumar Kajla

Investigations on cellular nanoparticles required for synthesis of chitin the precursor for chitosan Dissertation zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) an der Universität Osnabrück vorgelegt von Mayur Kumar Kajla aus Himachal Pradesh, Indien 2 0 0 5 September ii 1 .Berichterstatter : Prof. Dr. H. Schrempf 2. Berichterstatter : Prof. Dr. Jürgen Hofemeister iiiIndex P age 1.0 Introduction 1 2.0 Material and methods 7 2.1 Materials 7 2.1 Media 7 2.1.1 Yeast peptone adenine dextrose (YPAD) 7 2 Yeast malt extract (YM) 7 3 Luria Burtani (LB) 8 4 M9 Minimal Medium 8 2.2 Buffers and stains 9 2.2.1 Standard buffers 9 2 Western transfer buffer 9 3 Acetonitrile buffer 9 4 Buffers for blue native PAGE 10 5 Protein loading buffer for SDS-PAGE 10 6 Coomassie staining solution 10 7 Silver and zinc staining of polyacrylamide gels 11 7.1 Silver staining 11 2 Zinc staining 12 2.3 E. coli and S. cerevisiae strains 12 2.4 Antibodies 13 2.5 Biochemical methods 14 2.5.1 Cultivation of yeast 14 2 Chitin synthase assay 14 2.