Investigations of the intracellular localization and the function of sulfurtransferases in higher plants [Elektronische Ressource] / von Michael Bauer

Investigations of the intracellular localization and the function of sulfurtransferases in higher plants Dem Fachbereich Biologie der Universität Hannover zur Erlangung des Grades Doktor der Naturwissenschaften Dr. rer. nat. genehmigte Dissertation von Dipl.-Biol. Michael Bauer geboren am 12.01.1974 in Hannover 2004 REFERENTIN: PRIV.-DOZ. DR. J. PAPENBROCK KORREFERENT: Prof. Dr. T. Leeb Tag der Promotion: 11.06.2004 SUMMARY Sulfurtransferases/rhodaneses (Strs) comprise a group of enzymes widely distributed in archaea, eubacteria, and eukaryota, which catalyze the transfer of a sulfur atom from suitable sulfur donors to nucleophilic sulfur acceptors. In all the organisms analyzed to date small gene families encoding Str proteins have been identified. In this work Strs encoded in the nuclear genome of Arabidopsis thaliana were analyzed. 18 proteins were identified in Arabidopsis which contain at least one Str signature and were classified into six groups according to their sequence homology. Group I consists of two two-domain Str proteins (AtStr1 and AtStr2), while the proteins in group VI (AtStr14-18) contain only the C-terminal Str signature. Both two-domain proteins from group I showed higher affinity to 3-mercaptopyruvate as substrate than to thiosulfate.