Interplay between the transmembrane nucleoporin Pom121 and the Ran GTPase system [Elektronische Ressource] / presented by Emine Sevil Yavuz

Dissertation submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto-Carola University of Heidelberg, Germany for the degree of Doctor of Natural Sciences presented by Emine Sevil Yavuz born in: Isparta, TURKEY Oral-examination:: ................................................ 1 Interplay between the transmembrane nucleoporin Pom121 and the Ran GTPase system Referees: Dr. Jan Ellenberg Prof. Ed Hurt 2 Table of contents Summary 4 Zusammungfassung 5 Acknowledgements 6 List of figures 8 Abbreviations 9 Chapter 1 Introduction 11 1.1 Architecture of nuclear envelope 12 1.1.1 Nuclear pore complexes 12 1.1.2 Inner and outer nuclear envelope 15 1.2 The RanGTPase system and its implications 18 1.2.1 The small GTPase Ran 18 1.2.2 RanGEF 19 1.2.3 RanGAP 20 1.2.4 Transport receptors 21 1.2.5 Functions of RanGTPase system thoughout cell cycle 23 1.3 Aims of this thesis 29 Chapter 2 Materials and Methods 30 2.1 Materials 31 2.1.1 Reagents 31 2.1.2 Buffers and solutions 33 2.1.3 Primers and oligonucleotides 34 2.1.4 Antibodies 35 2.1.5 Bacterial strains 36 2.2 Methods 36 2.2.1 Molecular cloning 36 2.2.2 SDS-PAGE and Immunoblotting 44 2.2.