Internalization and photoinduced endosomal release of polyplexes studied on a single cell level [Elektronische Ressource] / Karla Gerda de Bruin

Dissertation zur Erlangung des Doktorgradesder Fakulta¨t fur¨ Chemie und Pharmazieder Ludwig Maximilians Universitat Munchen¨ ¨Internalization and PhotoinducedEndosomal Release of PolyplexesStudied on a Single Cell LevelKarla Gerda de BruinausAlmelo, Niederlande2008ErklärungDiese Dissertation wurde im Sinne vonx 13 Abs. 3 der Promotionsordnung vom 29. Januar1998 von Herrn Prof. Dr. Christoph Bräuchle betreut.Ehrenwörtliche VersicherungDiese Dissertation wurde selbständig, ohne unerlaubte Hilfe erarbeitet.München, den 2008Dissertation eingereicht am1. Gutachter: Prof. Dr. Christoph Bräuchle2. Prof. Dr. Ernst WagnerMündliche Prüfung am 3. Juli 2008Bobby McFerrinSummaryIn order to develop more ecient gene vectors a detailed understanding of the intracellularbehavior of these vectors is essential. Single particle tracking is a method that has proveduseful to elucidate biological processes on a single cell level. In this thesis single particletracking was used to study the internalization, intracellular tracking, and endosomal releaseof nonviral gene vectors consisting of plasmid DNA condensed with cationic polymers, so called polyplexes. A dual color setup allowed separate detection of either polyplex andGFP-labeled cellular structures or polymer and DNA.In the first part of this work the internalization of dierent polyplexes into the cell was stud ied.