Interaction of transient receptor potential Vanilloid 1 (TRPV1) with G-protein coupled receptors and TRP ion channels [Elektronische Ressource] / vorgelegt von Viola Spahn

Interaction of Transient Receptor Potential Vanilloid 1 (TRPV1) with G-protein coupled receptors and TRP ion channels Inauguraldissertation zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) am Fachbereich Biologie, Chemie, Pharmazie der Freien Universität Berlin vorgelegt von Viola Spahn Berlin 2011 Erstgutachter: Herr Prof. Dr. Christian Zöllner Zweitgutachterin: Frau Prof. Dr. Monika Schäfer-Korting Tag der Disputation: 28.01.2011 Table of contents Table of contents Abbreviations…………………...……………………...1 1. Introduction……….………………...………………....4 1.1. Pain………...……………………….………………...….……..5 1.2. Transient receptor potential ion channel family………...…..6 1.2.1. TRPV1…………………………………………...……………………….7 1.2.2. Sensitization of TRPV1………………………………..………………...9 1.2.3. TRPA1………………...……………………………...…………………11 1.3. Opioids……………………….…………………...…………...14 1.3.1. μ-opioid receptor……………………………..………………………...15 1.3.2. Opioid withdrawal-induced hyperalgesia………….…...…………….17 2. Objectives……………………………...……………...19 3. Animals, material and methods………….…………..20 3.1. Materials……………..……………………………….…….…20 3.1.1. Cell lines and bacteria…………………………………..…...…………20 3.1.2. Animals and animal housing………………………………….……….20 3.1.3. Chemicals…………………………………...…………………………..20 3.1.4. Media, buffer………………………..………………………………….22 3.1.5. Reaction systems………………………..………………………….…...23 3.1.6.