Inhibition of postangioplasty restenosis using antisense approach [Elektronische Ressource] / Vadim Tchaikovski

Universität UlmAus der Abteilung Innere Medizin llLeiter: Prof. Dr. V. HombachInhibition of postangioplasty restenosis using antisense approachDissertation zur Erlangung des Doktorgrades der MedizinDer Medizinischen Fakultät der Universität UlmVorgelegt vonVadim Tchaikovskiaus BaranowitschiRepublic of Belarus2003Amtierender Dekan: Prof. Dr. R. MarreBerichterstatter: PD Dr. J. WaltenbergerBerichterstatter: Prof. Dr. Michael KühlTag der Promotion: 08.05.20032To my family with deep gratitude for their persistent support of my academicand scientific endeavors3Table of contents:Abbreviations and acronyms…………………………………………………….. 61 Introduction……………………………….……………………………..………... 8 1.1 Postangioplasty restenosis…………………………………………………... 8 1.1.1 Definition of restenosis………………………………………………….. 8 1.1.2 Mechanisms of restenosis…………………………………………….... 8 1.1.3 Animal models of restenosis and the role of vascular smooth muscle cells in restenosis……………………………………………… 11 1.2 Platelet-derived growth factor (PDGF)/ PDGF receptor (PDGFR) system and its role in restenosis……………………………………………. 12 1.2.1 Biochemistry of the PDGF/PDGFR system....................................... 12 1.2.2 Role of PDGF/PDGFR system in postangioplasty restenosis…….... 14 1.3 Antisense (AS) technology…………………………………………….…….. 15 1.3.1 AS oligonucleotide (ODN): mechanism of action………………….…. 16 1.3.