In vitro neurogenesis of adult neural stem cells from bone marrow and brain [Elektronische Ressource] / vorgelegt von Constantin Andreas Werner Hermann

Abteilung für Neurologie Leiter: Prof. Dr. A. C. Ludolph Universität Ulm In vitro neurogenesis of adult neural stem cells from bone marrow and brain Dissertation zur Erlangung des Doktorgrades Dr. rer. med. der Medizinischen Fakultät der Universität Ulm vorgelegt von Constantin Andreas Werner Hermann geboren in Backnang Ulm im April 2006 Amtierender Dekan: Prof. Dr. med. Klaus-Michael Debatin 1.Gutachter: Prof. Dr. med. Alexander Storch 2.Gutachter: Prof. Dr. rer. nat. Klaus-Dieter Spindler Tag der Promotion: 06. Februar 2007 - ii - Meiner Familie - iii - Table of Contents 1. Introduction 1 1.1. Neural stem cells: Origin and definition 1 1.2. Neural stem cells in culture 2 1.2.1. Brain-derived adult neural stem cells 3 1.2.2. Adult multipotent stem cells 3 1.3. Aim of this study 4 2. Results and Discussion 5 2.1. Epigenetic conversion of human adult bone marrow stromal cells into neural stem cells 5 2.2. Isolation, expansion and in vitro characterization of adult human hippocampal progenitor cells 8 2.3. Functional neuronal and dopaminergic differentiation of multipotent neural stem cells from the adult tegmentum 10 3. Summary 12 4. References 14 5.