Immunohistochemistry in diagnostic pathology of tumors [Elektronische Ressource] : approach, benefits, limits and pitfalls / von Muin Sami Ahmad Tuffaha

Aus dem Institut für Pathologie der Medizinischen Fakultät Charité – Universitätsmedizin Berlin DISSERTATION Immunohistochemistry in diagnostic pathology of tumors. Approach, benefits, limits and pitfalls zur Erlangung des akademischen Grades Doctor medicinae (Dr. med.) vorgelegt der Medizinischen Fakultät Charité – Universitätsmedizin Berlin von Muin Sami Ahmad Tuffaha aus Amman, Jordanien Gutachter/in: 1. Prof. Dr. med. H. Guski 2. Prof. Dr. med. G. Kristiansen 3. Prof. Dr. med. V. Krenn Datum der Promotion: 19.11.2010 Contents Page 1. Introduction 5 2. Aim of the study 6 3. Material and methods 7 3.1 Material 7 3.2 Antibodies 9 3.3 Methods 11 3.3.1 Immunohistochemical staining 11 3.3.2 Immunohistochemical double staining 13 4. Immunohistochemical pathways for the diagnosis of metastasis of unknown primary tumors 15 4.1 Diagnostic algorithms for tumor screening 16 5. Antibodies for immunohistochemical tumor diagnosis. Diagnostic approach targeting antigens with multilineal or atypical expression, benefits and pitfalls 25 5.1 Antibodies for the diagnosis of epithelial neoplasia 25 5.2 es for the diagnosis of pulmonary tumors 34 5.3 Antibodies for the diagnosis of gastrointestinal tumors 36 5.4 es for the diagnosis of exocrine pancreatic tumors 38 5.5 Antibodies for the diagnosis of liver tumors 39 5.6 es for the diagnosis of breast tumors 41 5.