Identification of amino acids within the substrate binding region of organic cation transporters (OCTs) that are involved in binding of corticosterone [Elektronische Ressource] / vorgelegt von Natalia Shatskaya

Identification of amino acids within the substrate binding region of organic cation transporters (OCTs) that are involved in binding of corticosterone. Dissertation zur Erlangung des naturwissenschaftlichen Doktorgrades der Bayerischen Julius-Maximilians-Universität Würzburg vorgelegt von Natalia Shatskaya aus Nowosibirsk Würzburg 2006 Eingereicht am: Mitglieder der Promotionskommission: Vorsitzender: Gutachter : Gutachter: Tag des Promotionskolloquiums: Doktorurkunde ausgehändigt am: 1. Introduction 1 1.1. Transporters of SLC22 family 2 1.2. Functional characteristics of OCT transporters 5 1.3. Substrate specificities of OCTs 7 1.4. The tissue distribution and cellular localization of OCT subtypes 11 1.5. Polymorphisms and mutations in OCTs 13 1.6. Clinical relevance of OCTs 17 2. The aim of study 20 3. Materials and Methods 21 3.1. Materials 21 3.1.1. Chemicals 21 3.1.2. Radioactive compounds 22 3.1.3. Enzymes and kits 22 3.1.4. Equipment 22 3.2. Methods of Molecular Biology 23 3.2.1. Wild type rOCT plasmids, construction of chimeras and point mutations 23 3.2.2. Linearization of plasmid DNA 25 3.2.3. Spectrophotometric analysis of DNA 25 3.2.4. DNA electrophoresis 26 3.2.5. Transcription of mRNA from Template DNA 26 3.2.6. RNA electrophoresis 27 3.3.