Identification, characterization and application of novel (R)-selective amine transaminases [Elektronische Ressource] / Sebastian Schätzle

Identification, characterization and application of novel (R)-selective amine transaminases Inauguraldissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) an der Mathematisch-Naturwissenschaftlichen Fakultät der Ernst-Moritz-Arndt-Universität Greifswald vorgelegt von Sebastian Schätzle geboren am 12.1.1983 in Duisburg Greifswald, November 2011 Dekan: Prof. Dr. Klaus Fesser 1. Gutachter: Prof. Dr. Uwe T. Bornscheuer 2. Gutachter: Prof. Dr. Per Berglund Tag der Promotion: 11.01.2012 The Contents TABLE OF CONTENTS TABLE OF CONTENTS I LIST OF ABBREVIATIONS AND SYMBOLS II SCOPE AND OUTLINE OF THIS THESIS III 1 THE BACKGROUND - 1 - 1.1 AMINES IN NATURE AND PHARMACEUTICALS - 2 - 1.2 CHEMICAL SYNTHESIS OF CHIRAL AMINES - 3 - 1.3 ENZYMATIC SYNTHESIS OF CHIRAL AMINES - 4 - 1.4 TRANSAMINASES – ENZYMES OF NOBLE FAMILY - 5 - 1.5 ENZYME DISCOVERY – MANY WAYS LEAD TO ROME ARTICLE I - 7 - 2 THE ANALYTICS - 9 - 2.1 A FAST AND EASY ASSAY FOR SCREENING ARTICLE II - 11 - 2.