Identification and characterization of miRNA-133b as a novel regulator of death receptor mediated apoptosis [Elektronische Ressource] / von Juan Pablo Patrón Arcila

Identification and characterization of miRNA-133b as a novel regulator of death receptor mediated apoptosis Dissertation zur Erlangung des akademischen Grades Doctor rerum naturalium (Dr. rer. nat.) im Fach Biologie eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät I der Humboldt Universität zu Berlin von Diplom-Biologe Juan Pablo Patrón Arcila Präsident der Humboldt-Universität zu Berlin Prof. Dr. Dr. h.c. Christoph Markschies Dekan der Mathematisch-Naturwissenschaftlichen Fakultät I Prof. Dr. Lutz-Helmut Schön Gutachter/-innen: 1.) Prof. Dr. Dr. h.c. Stefan H. Kaufmann 2.) Prof. Dr. Arturo Zychlinsky 3.) Prof. Dr. Michael U. Martin Tag der mündlichen Prüfung: 26.07.2010 "The most exciting phrase to hear in science, the one that heralds the most discoveries, is not "Eureka!" (I found it!) but 'That's funny...'" — Isaac Asimov II Table of contents Summary VI Zusammenfassung VIII Abbreviations X 1. Introduction 1 1.1 MicroRNAs 1 1.1.1 History 1 1.1.2 miRNA biogenesis 2 1.1.3 Mechanisms of miRNA-mediated gene regulation 3 1.2 Apoptosis 5 1.2.1 Extrinsic apoptotic pathway 5 1.2.2 Intrinsic apoptotic pathway 6 1.2.3 Crosstalk between apoptosis signalling pathways 8 1.2.4 Negative regulators of apoptosis 9 1.2.5 miRNA-mediated regulation of apoptosis 10 1.3 Pathogen recognition during innate immunity 11 1.3.