How kinesin-1 deals with roadblocks [Elektronische Ressource] : biophysical description and nanotechnological application / by Till Korten

How Kinesin-1 Deals WithRoadblocks: BiophysicalDescription andNanotechnological Applicationdissertationsubmitted for the degree ofDoctor rerum naturalium(Dr. rer. nat.)at theFakult at fur Mathematik und NaturwissenschaftenTechnische Universit at DresdenbyTill Kortenthborn on April 6 , 1978 in Berlin, GermanythSeptember 30 , 20091. Reviewer: Prof. Dr. Anthony Hyman2. Reviewer: Prof. Dr. Henry HessthDay of the defense: December 10 2009Signature from head of PhD committee:AbstractProteins have been optimized by evolution for billions of years to work on ananometer scale. Therefore, they are extremely promising for nanotechnologicalapplications. Cytoskeletal laments propelled by surface-attached motor proteinshave been recently established as versatile transport platforms for nano-sized cargoin molecular sorting and nano-assembly devices. However, in this gliding motilitysetup, cargo and motors share the lament lattice as a common substrate for theiractivity. Therefore, it is important to understand the in uence of cargo-loadingon transport properties.By performing single molecule stepping assays on biotinylated microtubules, itwas shown that kinesin-1 motors rst stop and then detach when they encountera streptavidin obstacle on their path along the microtubule.