Global analysis of cellular protein dynamics by pulse-labeling and quanti tati ve mass spectrometry [Elektronische Ressource] / Björn Schwanhäußer. Gutachter: Thomas Sommer ; Matt hias Selbach ; Andreas Herrmann

Global analysis of cellular protein dynamics by pulse-labeling and quan ta ve mass spectrometryDissertationzur Erlangung des akademischen Gradesdoctor rerum naturalium (Dr. rer. nat.)im Fach Biologieeingereicht an der Mathema sch-Naturwissenscha lichen Fakultät Ider Humboldt-Universität zu Berlinvon Dipl.-Biol. Björn Schwanhäußer Präsident der Humboldt-Universität zu BerlinProf. Dr. Dr. h.c. Christoph MarkschiesDekan der Mathema sch-Naturwissenscha lichen Fakultät IProf. Dr. Andreas HerrmannGutachter/innen: 1. Prof. Thomas Sommer 2. Prof. Ma hias Selbach 3. Prof. Andreas HerrmannTag der mündlichen Prüfung: 23.02.2010tttitiftfttitifor LiamTable of contentsTABLE OF CONTENTS iABSTRACT vZUSAMMENFASSUNGiABBREVIATIONSiiI INTRODUCTION 12I.1. Mass spectrometry in a nutshell 13I.1.1. Instrumenta on and workfl ow of mass spectrometry 13I.1.2. Quan ta ve approaches in mass spectrometry-based proteomics 17I.1.2.1 Rela ve MS-based quan fi ca on with heavy stable-isotopes 17I.1.2.2 Stable isotope labeling by amino acids in cell cultue (SILAC) 19I.1.2.3 Absolute quan fi ca on of proteins 21I.1.2.4 Label-free approaches 21I.2. Biological implica ons of mass spectrometry-based quan ta ve proteomics 23I.2.1. Transla onal regula on of gene expression 23I.2.2. Regula on of gene expression exerted by microRNAs 23I.2.3. The complex rela onship between mRNAs and proteins 25I.3.