Genetically encoded calcium indicators based on troponin C and fluorescent proteins [Elektronische Ressource] / vorgelegt von Nicola Heim

GENETICALLY ENCODED CALCIUM INDICATORS BASED ON TROPONIN C AND FLUORESCENT PROTEINS Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften an der Fakultät für Biologie der Ludwig-Maximilians-Universität München vorgelegt von Nicola Heim München, 2005 Erstgutachter: Prof. Dr. Alexander Borst Zweitgutachter: Prof. Dr. Rainer Uhl Tag der mündlichen Prüfung: 24. Oktober 2005 VTABLE OF CONTENTS TABLE OF CONTENTS..........................................................................................................V 1. ABSTRACT....................................................................................................................1 2. INTRODUCTION ............................................................................................................3 2.1 Fluorescence Techniques....................................................................................3 2.1.1 Fluorescence and FRET................................................................................3 2.1.2 The Green Fluorescent Protein .....................................................................8 2.1.3 Fluorescent Calcium Probes .......................................................................11 2.2 Calcium-Binding Proteins...................................................................................15 2.2.1 EF-Hands..........................