Genetic diversity and phenotypic characterization of HIV-1 circulating recombinant forms (CRFs) among drug-naïve and -exposed patients in Burkina Faso [Elektronische Ressource] / presented by Kanokporn Sathiandee

Dissertation submitted to the Combined Faculties for the Natural Sciences and for Mathematics of the Ruperto-Carola University of Heidelberg, Germany for the degree of Doctor of Natural Sciences presented by M.Sc. Kanokporn Sathiandee born in Sakon Nakhon, Thailand Oral examination: November 27, 2009 Genetic diversity and phenotypic characterization of HIV-1 Circulating Recombinant Forms (CRFs) among drug-naïve and -exposed patients in Burkina Faso Referees: Prof. Dr. Hans-Georg Kräusslich Prof. Dr. Valerie Bosch Summary Summary The genotypic and phenotypic characteristics of HIV-1 CRF02_AG strains from infected patients in Burkina Faso were determined in order to understand about resistance development and susceptibility to antiretroviral drugs of HIV-1 non-subtype B. Drug resistance studies among Nevirapine (NVP) naïve and exposed women from Nouna, and HAART-exposed HIV patients attending the University Hospital in Ouagadougou, Burkina Faso were performed. The diversity and evolution of HIV-1 reverse transcriptase (RT) quasispecies in paired plasma, breast milk whey and/or breast milk cells from seventeen HIV-infected NVP-naïve and exposed women were determined by direct sequencing and clonal analysis. NVP resistance mutations were detected in 8% of infected mothers by direct sequencing.