Generation of tools to investigate Chikungunya virus [Elektronische Ressource] / vorgelegt von Vu Xuan Nghia

Aus dem Institut für Virologie und ImmunbiologieDer Universität WürzburgVorstand: Professor Dr. med. Axel RethwilmGeneration of tools to investigate Chikungunya virusInaugural – DissertationZur Erlangung der Doktorwürde derMedizinischen FakultätderBayerischen Julius-Maximilians-Universität zu Würzburgvorgelegt vonVu Xuan Nghiaaus VietnamWürzburg, Juli 2008 Referent: Prof. Dr. A. RethwilmKorreferent: Prof. Dr. K. BrehmDekan: Prof. Dr. Matthias FroshTag der mündlichen Prüfung:Der Promovend ist ArztI dedicate my thesis to my wife and my sonsCONTENTSPage1. Introduction 11.1. Chikungunya virus and re-emergence of Chikungunya virus 11.2. The genome and replicative cycle of CHIKV 31.2.1. Genome structure and organization 31.2.2. The replication-cycle of CHIKV 61.3. CHIKV clinical manifestation 71.4. The development of antiviral drugs and tools of viral diagnosis 91.5. The aim of thesis 102. Materials 112.1. Enzymes and Buffers 112.2. Antibiotics 112.3. Competent cells 112.4. Antibodies 112.5. Vectors 122.6. Oligonucleotids 122.7. Kits 132.8. Others 132.9. Media, solutions and buffers 142.9.1. Bacteria cultures 142.9.2. Buffers and solutions for analysis and cloning DNA 142.9.3. Buffers and solutions for protein analysis 152.10. Standard solutions and buffers 192.11. Blood samples 202.11.1. Blood sample control 202.11.2. CHIKV patient’s sera 202.11.3. Vietnamese donor blood samples 203. Methods 213.1. Make competent E.coli cells 213.