Generation and characterization of MAPKAPK5 deficient mice [Elektronische Ressource] / von Yu Shi

Generation and Characterization of MAPKAPK5-Deficient MiceDissertationvon der Fachbereich Chemieder Universität Hannoverzur Erlangung des Grades einerDOKTORS DER NATURWISSENSCHAFTENDr. rer. nat.genehmigte Dissertationvon MSc. Yu Shigeboren am 01. Mai 1963, in Heilongjiang, V.R. China2002PDF created with FinePrint pdfFactory trial version http://www.fineprint.comReferent: Prof. Dr. H. MeyerKorreferent: Prof. Dr. M. Gaestel, Prof. Dr. E. UngewickellTag der Promotion: 29. Oktober 20021PDF created with FinePrint pdfFactory trial version http://www.fineprint.comAbstractThe p38 mitogen-activated protein kinase (MAPK) pathway, like the c-Jun N-terminalkinase (JNK) MAPK pathway, is activated in response to cellular stress andinflammation and is involved in many fundamental biological processes. MAPKAPkinase 5 (MK5) is one of several kinases that are regulated through directphosphorylation by p38 MAPK. To study the role of the p38 MAPK pathway, and thefunction of MK5 in vivo, we have generated mice with a germline mutation of the MK5gene. The mice have been characterised by Southern blot analysis, RT-PCR,sequencing, as well as Western blot analysis and protein kinase assays, which alldemonstrated that the targeted disruption of the MK5 gene resulted in a null allele.MK5-deficient mice were viable and fertile.