Gene expression profiling and gene regulation for functional genomics in mouse models [Elektronische Ressource] / vorgelegt von Johannes Beckers

Gene expression profiling and gene regulation for functional genomics in mouse models H a b i l i t a t i o n s s c h r i f t vorgelegt von J o h a n n e s B e c k e r s Lehrstuhl für Experimentelle Genetik Department für Biowissenschaftliche Grundlagen Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt Technische Universität München Freising Juni 2007 Expression genomics in mice CONTENTS 1. Summary 5 2. Introduction 7 2.1. Positioning of past and current research in the field. Why study gene expression? 2.2. Gene regulation increases genome complexity. A theoretical consideration. 2.3. Gene products as multi-functional tools. Gene regulation as integral part of gene function. 2.4. The presented research in a nutshell. From systematic expression-profiling to single gene functional approaches. 3. Research 13 Introduction 3.1. Molecular phenotyping of mouse tissues using microarray based transcript profiling 3.1.1. DNA-microarrays for gene expression-profiling. From the emergence of the technology to its clinical application. 3.1.2. A story of success. Application of microarray based expression-profiling for mouse tissues. Own research 17 3.1.3. Establishing a high quality microarray platform for gene expression-profiling. 3.1.4. A novel method to experimentally assess the specificity of nucleic acid hybridisation in situ on the microarray surface. 3.1.5.