Functional implications of bone morphogenetic protein 10 (BMP10) expression in pathological hearts [Elektronische Ressource] / vorgelegt von Izabela Piotrowska

Functional implications of Bone Morphogenetic Protein 10 (BMP10) expression in pathological hearts Inauguraldissertation zur Erlangung des Grades einesDoctors der Humanbiologie Des Fachbereichs Medizin Der Justus-Liebig-Universität Gieβen vorgelegt von Izabela Piotrowska aus Poznań, Polen Gieβen 2006 Aus dem Max-Planck-Institute für Hertz und Lungen Forschung In Bad Nauheim Direktor: Prof. Dr. Thomas Braun Gutachter: Prof. Dr. T. Braun Gutachter: Prof. Dr. G. Euler Tag der Disputation: 2 July 2007 1. Introduction 1 1.1. Bone Morphogenetic Proteins as members of Transforming Growth Factor β Superfamily 1 1.2. BMPs receptors 2 1.3. Smad-dependent and Smad-independent signaling pathways 4 1.4. Role of BMP signaling in heart development and angiogenesis 6 1.5. BMP10 as a heart specific member of the TGFβ superfamily 11 1.6. Aim of the project 12 2. Experimental procedures 13 2.1. Materials 13 2.1.1. Basic materials 13 2.1.2. Chemicals 13 2.1.3. Radiochemicals 14 2.1.4. Reagents 14 2.1.5. Enzymes 16 2.1.6. Kits 16 2.1.7. Oligonucleotides 17 2.1.8. Vectors and Plasmids 19 2.1.8.1. Plasmids for riboprobes synthesis 20 2.1.9. Bacterial strains 20 2.1.10. Cell lines 21 2.1.11.