Functional genomic approaches to analyse the parasitic interaction between the model legume Medicago truncatula and the oomycete Aphanomyces euteiches [Elektronische Ressource] / von Frank Colditz

Functional genomic approaches to analyse the parasitic interaction between the model legume Medicago truncatula and the oomycete Aphanomyces euteiches Von dem Fachbereich Biologie der Universität Hannover zur Erlangung des Grades eines Doktors der Naturwissenschaften Dr. rer. nat. genehmigte Dissertation von Dipl.-Biol. Frank Colditz geboren am 31.05.1971 in Großburgwedel 2005 Referentin: PD Dr. Franziska Krajinski Korreferent: Prof. Dr. Hans-Peter Braun Tag der Promotion: 03.02.2005 Abstract Abstract The common root rot caused by the oomycete Aphanomyces euteiches is a major yield-reducing factor in legume crop production, and it is considered to be the most destructive disease of pea in areas with temperate climates. Disease development with discolored lesions, a watery rotting of root tissue and a significant reduction of root mass are typical symptoms and is well-characterized, but so far very little is known about the molecular mechanisms of the disease and the nature of host cellular responses. Comparative functional genomic approaches were carried out to systematically identify plant genes and proteins that show altered regulation in the model legume Medicago truncatula after A. euteiches infection. A SSH-cDNA library was established that revealed 51 cDNAs to be strongly induced in infected roots.