Functional characterization of the Ca_1hn2_1hn+-regulated protein kinase AtCIPK8 involved in ABA and oxidative stress responses [Elektronische Ressource] / vorgelegt von Iuliia Knutova

Biologie2+Functional characterization of the Ca -regulated protein kinaseAtCIPK8 involved in ABA and oxidative stress responsesInaugural-Dissertationzur Erlangung des Doktorgradesder Naturwissenschaften im Fachbereich Biologieder Mathematisch-Naturwissenschaftlichen Fakultat¨der Westfalischen Wilhelms-Universitat Munster¨ ¨ ¨vorgelegt vonIuliia Knutovaaus Vinnitsya, Ukraine.2008Dekan: Prof. Dr. C. Klam¨ btErster Gutachter: Prof. Dr. J. KudlaZweite Gutachterin: Prof. Dr. A. von SchaewenTag der mundlic¨ hen Prufung:¨ 19.01.2009Tag der Promotion: 6.02.2009ContentsAbbreviations 11 Introduction 31.1 Calcium as a second messenger ........................ 31.2 Decoding the calcium message ......................... 41.2.1 Calciumsensors............................. 51.3 CBL-interacting protein kinases (CIPKs) ................... 81.3.1 Activation mechanism of CIPKs .................... 81.3.2 Biochemical properties of 101.3.3 Subcellular distribution of CIPKs 121.3.4 Physiological functions of 121.4 Reactiveoxygenspeciesassecondmessengers................ 151.4.1 Sources of ROS ............................. 151.4.2 The antioxidant system......................... 211.5 Generation of ROS under stress conditions .................. 241.6 Aimsofthisthesis ............................... 272 Materials and Methods 292.1 Polymerase Chain Reaction (PCR) ...................... 292.1.1 Primer design .............................. 292.1.