Functional characterisation of two channels proteins involved in leguminous symbiosis [Elektronische Ressource] / vorgelegt von Myriam Charpentier

Functional Characterisation of Two Channels Proteins Involved in Leguminous Symbiosis Dissertation der Fakultät für Biologie der Ludwig-Maximilians-Universität München vorgelegt von Charpentier Myriam München im September 2008 Dekan: Prof. Dr. Jürgen Soll Gutachter: Prof. Dr. Martin Parniske Gutachter: Prof. Dr. Heinrich Jung Datum der Disputation: 18.12.2008 2 To my father (194 9- 199 9 ) 3 Table of contents List of abbreviations 7 List of Units 9 Abstract 10 Zusammenfassung 11 1. Introduction 12 1.1 Endosymbiotic plant-microbe interactions 12 1.2 Root-nodule-symbiosis early ion fluxes 17 1.3 Dissection of the calcium-spiking pathway 22 1.4 Castor and pollux mutant and aim of the study 23 2. Results 28 2.1 CASTOR and POLLUX identification 28 2.2 Sequence analyses 31 2.3 Nuclear localization of CASTOR and POLLUX 35 2.4 Homodomerization of CASTOR and POLLUX 40 2.5 Expression of POLLUX and DMI1 under the control of P35S restore the nodulation phenotype of castor-12 mutant 43 2.6 Functional characterization 47 2.6.1 Yeast complementation assays 47 2.6.1.