Functional characterisation of NIC2, a member of the MATE family from Arabidopsis thaliana (L.) Heynh. [Elektronische Ressource] / von Blazej Dolniak

Institut für Biochemie und Biologie Functional characterisation of NIC2, a member of the MATE family from Arabidopsis thaliana (L.) Heynh. Dissertation zur Erlangung des akademischen Grades "doctor rerum naturalium" (Dr. rer. nat.) in der Wissenschaftsdisziplin "Molekularbiologie" eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät der Universität Potsdam von Blazej Dolniak Potsdam, den 28.02.2005. najblizszym mi osobom Contents Introduction 1 1. The Multidrug efflux pumps 1 2. Multidrug and toxic compounds extrusion family 4 2.1. Bacterial multidrug and toxic compound extrusion family 5 2.2. Yeast multidrug and toxic compound extrusion family 8 2.3. Plant multidrug and toxic compounds extrusion family 9 • Arabidopsis thaliana Novel Ion Carrier (NIC)-family 11 • Identification of MATE genes in other plant species 12 Aim ofwork 14 Materials and methods 15 1. General materials and methods 15 2. Escherichia coli-expression host system 15 - Bacterial strains 5 - Cloning of NIC2 and YDHE/NORE 16 - Media and growth conditions 17 - Drug susceptibility assays 17 3.