Functional analysis of the sucrose synthase gene family in Arabidopsis thaliana [Elektronische Ressource] / von Zuzanna Bieniawska

Dissertation Functional analysis of the sucrose synthase gene family in Arabidopsis thaliana vorgelegt an der Mathematisch-Naturwissenschaftlichen Fakultät der Universität Potsdam von Zuzanna Bieniawska geb. am 12.04.1978 in Wroclaw (Polen) zur Erlangung des akademischen Grades Dr. rer. nat. Wissenschaftsdisziplin: Molekularbiologie der Pflanzen 6. März 2006 Cz łowiek mo że znieść bardzo du żo, lecz pe łnia błąd s ądz ąc, że potrafi znieść wszystko Fiodor Dostojewski Abstract Abstract Sucrose synthase (Susy) is a key enzyme of sucrose metabolism, catalysing the reversible conversion of sucrose and UDP to UDP-glucose and fructose. Therefore, its activity, localization and function have been studied in various plant species. It has been shown that Susy can play a role in supplying energy in companion cells for phloem loading (Fu and Park, 1995), provides substrates for starch synthesis (Zrenner et al., 1995), and supplies UDP-glucose for cell wall synthesis (Haigler et al., 2001). Analysis of the Arabidopsis genome identifies six Susy isoforms. The expression of these isoforms was investigated using promoter-reporter gene constructs (GUS) and real time RT-PCR.