Fluorescence microscopy studies on the fibrillation of IAPP at model and cellular lipid interfaces, from mechanism to potential strategies in type II diabetes mellitus [Elektronische Ressource] / von Diana Radovan

Fluorescence microscopy studies on the fibrillation of IAPP at model and cellular lipid interfaces - from mechanism to potential strategies in type II diabetes mellitus Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften (Dr. rer. nat.) eingereicht beim Fachbereich Chemie der Technische Universität Dortmund von M.Sc. BMB Diana Radovan aus Timi şoara, Rumänien 2009 Dortmund First Referee: Prof. Dr. R. Winter Second Referee: Prof. Dr. H. Rehage 2 Acknowledgements I would first of all like to express my gratitude towards Prof. Dr. Roland Winter for giving me the chance to further develop my interest in lipid research and cytotoxicity tests, but also to become familiar with and get specialized in the fascinating area of amyloidogenic proteins and diseases, as well as in advanced imaging techniques. I would particularly like to thank Dr. Norbert Opitz from MPI Dortmund, for his help and experimental support in performing the confocal and two-photon excited microscopy experiments. Thanks to Dr. Claus Csezlik for his constant experimental advice and useful discussions. I would also like to thank Dr. Nagarajan Periasamy for guiding my first steps in the field of GUVs, Andrea Gohlke who was actively co-involved in the development of the cell culture lab, Dr. Rajesh Mishra for many useful discussions in the field of amyloids, Dr.