Fluorescence imaging microscopy studies on single molecule diffusion and photophysical dynamics [Elektronische Ressource] / vorgelegt von Stephan Schäfer

KInstitut fur˜ BiophysikFachrichtung fur˜ PhysikFakult˜at fur˜ Mathematik und NaturwissenschaftenTechnische Universit˜at DresdenFluorescence imaging microscopy studieson single molecule difiusion andphotophysical dynamicsDissertationzur Erlangung des Akademischen GradesDoktor rerum naturalium(Dr. rer. nat.)vorgelegt vonStephan Sch˜afergeboren in Limburg/Lahn am 9. August 1973September 20061. Gutachter: Prof. Dr. Petra Schwille2. Gutachter: Prof. Dr. Lukas Eng3. Gutachter: Prof. Dr. Ulrich KubitscheckDas Rigorosum und die Disputation fanden am 9. M˜arz 2007 statt.Meinen Eltern und meiner Gro…mutter.3AbstractWithin the last years, e.g. by investigating the uorescence of single moleculesin biological cells, remarkable progress has been made in cell biology extendingconventional ensemble techniques concerning temporal / spatial resolution andthe detection of particle subpopulations [82]. In addition to employing single uorophores as "molecular beacons" to determine the position of biomolecules,single molecule uorescence studies allow to access the photophysical dynamics ofgenetically encoded uorescent proteins itself.However, in order to gain statistically consistent results, e.g. on the mobilitybehavior or the photophysical properties, the uorescence image sequences haveto be analyzed in a preferentially automated and calibrated (non-biased) way.