Expression and function of the chemokine receptor XCR1 on murine CD8_1hn+ DC [Elektronische Ressource] / von Ahmed Mora

Dissertation Expression and function of the chemokine +receptor XCR1 on murine CD8 DC zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) im Fach Biologie eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät I der Humboldt-Universität zu Berlin von Master Biochemiker Ahmed Mora geboren am 27.12.1978 in Lyon Präsident der Humboldt-Universität zu Berlin Prof. Dr. Dr. h.c. Christoph Markschies Dekan der Mathematisch-Naturwissenschaftlichen Fakultät I Prof. Dr. Lutz-Helmut Schön Gutachter: 1. Prof. Dr. Peter-Michael Kloetzel 2. Prof. Dr. Richard Kroczek 3. Dr. Michal Or-Guil Tag der mündlichen Prüfung:16-03-2010 Summary The G protein-coupled receptor XCR1 has been described as the sole receptor for the chemokine ATAC. As contradictory data were published on the expression pattern of XCR1, its role in the immune system has not yet been defined. +/+In this work, expression of XCR1 was characterized in B6.XCR1-lacZ reporter mice which express β-galactosidase under the control of the XCR1 promoter. In tissue sections, strong expression of XCR1 was only detected in lymphoid organs like spleen, lymph nodes +and thymus. In the spleen, XCR1 cells were mainly found in the marginal zones, but also in the red pulp and the T cell zones. Flow cytometric analysis demonstrated exclusive +expression of XCR1 on DC, mainly on the CD8 DC subset, but also on a minority of − − +CD4 CD8 DC.