Expression and function of protease-activated receptors in human monocyte-derived dendritic cells [Elektronische Ressource] / submitted by Svetlana Paskas

University of Ulm Institute of Pharmacology, Toxicology and Natural Products Department of Pharmacology of Natural Products and Clinical Pharmacology Head: Prof. Dr. Th. Simmet Expression and Function of Protease-activated Receptors in Human Monocyte-derived Dendritic Cells Thesis Presented to the Faculty of Medicine, University of Ulm, to obtain the degree of a Doctor of Human Biology Submitted by Svetlana Paskas from Belgrade 2005 Amtierender Dekan: Prof. Dr. Klaus-Michael Debatin 1. Berichterstatter: Prof. Dr. Thomas Simmet 2. Berichterstatter: Dr. Jan Torzewski Tag der Promotion: 17.02.2006. 2 Abbreviations 6 Abstract 9 1. Introduction 10 1.1. Dendritic cells1.1.1. Origin and heterogeneity of dendritic cells 10 1.1.2. Maturation of dendritic cells 12 1.1.3. Dendritic cell immunobiology 15 1.1.4. Clinical implications of dendritic cells 16 1.1.5. Dendritic cells in atherosclerosis 17 1.2. Protease-activated receptors (PARs) 18 1.2.1. The roles of thrombin1.2.2. Protease-activated receptor 1 19 1.2.3. Protease-activated receptor 2 22 1.2.3. Protease-activated receptor 31.2.4. Protease-activated receptor 4 23 1.2.5. Signal transduction of protease-activated receptors1.2.6. PAR1 and PAR3 promoters 25 1.3. The aim of the work 28 2. Materials and Methods 29 2.1. Materials2.1.1. Cell lines2.1.2. Bacteria2.1.3. Antibodies2.1.4.