Elucidation of thiol protein oxidoreductase activity of the cytokine macrophage migration inhibitory factor (MIF) by biochemical redox and site specific mutagenesis analysis [Elektronische Ressource] / vorgelegt von Nguyen Tuyet Mai

Elucidation of thiol-protein oxidoreductase activity of the cytokine macrophage migration inhibitory factor (MIF) by biochemical redox and site-specific mutagenesis analysis Von der Fakultät Geo- und Biowissenschaften der Universität Stuttgart genehmigte Abhandlung zur Erlangung der Würde eines Doktors der Naturwissenschaften (Dr. rer. nat.) vorgelegt von Nguyen Tuyet Mai aus Hanoi, Vietnam Hauptberichter: Prof. Dr. Peter Scheurich Mitberichter: Prof. Dr. Jürgen Bernhagen Tag der mündlichen Prüfung: 31. Januar 2003 Institut für Grenzflächenverfahrenstechnik Universität Stuttgart 2003 Brief Contents Acknowledgements Abbreviations Contents Sumary 1 Zusammenfassung 5 Introduction 8 Materials and Methods 26 Results 63 Discussion 113 References 125 Publications Curriculum Vitae Declaration Acknowledgements • Acknowledgements I would like to sincerely thank Prof. Dr. Peter Scheurich for supervising this thesis, for helpful discussions and evaluating this work. I would like to express my sincere gratitude to Prof. Dr. Jürgen Bernhagen not only for his supervision and support of this thesis, but also for kindly helping me with my stay in Germany.