Electrophysiological and molecular analysis of aminergic neurons controlling arousal [Elektronische Ressource] / vorgelegt von Boris Klyuch

Electrophysiological and molecular analysisof aminergic neurons controlling arousalInaugural-DissertationzurErlangung des Doktorgrades derMathematisch-Naturwissenschaftlichen Fakultätder Heinrich-Heine-Universität Düsseldorfvorgelegt vonBoris Klyuchaus PetropavlovskJuni, 2008Aus dem Institut für Institute of Neurophysiologyder Heinrich-Heine Universität DüsseldorfGedruckt mit der Genehmigung derMathematisch-Naturwissenschaftlichen Fakultät derHeinrich-Heine-Universität DüsseldorfReferent: Prof. Dr. Helmut L. HaasKoreferent: Prof. Dr. Christine RoseTag der mündlichen Prüfung: Juni, 2008SummaryOne of the most important roles of histaminergic and dopaminergic systems isregulation of sleep-waking cycle (Brown et al., 2001). In my thesis I and colleagues showedthe effect of several well-known wake (or sleep) - promoting neurotransmitters and substanceon the activity of HAergic and DAergic neurons.Adenosine does not change the firing rate or membrane potential of TMN neurons inhypothalamic brain slices (Sergeeva et al., 2006). These results support the idea about indirectmodulation of HAergic systems with adenosine. In contrast, ATP, ADP and UTP excite TMNneurons in hypothalamic slices (Sergeeva et al., 2006). With a help of expression analysis wefound that P2Y and P2Y receptors are the major target for purines in TMN neurons. The1 4 other point of this work is found interactions between TMN neurons and glial cells (Sergeevaet al., 2006).