Electron multiplying CCD [Elektronische Ressource] : based detection in Fluorescence Correlation Spectroscopy and measurements in living zebrafish embryos / vorgelegt von Markus Burkhardt

Institut fur¨ BiophysikFachrichtung PhysikFakultat¨ fur¨ Mathematik und NaturwissenschaftenTechnische Universitat¨ DresdenElectron multiplying CCD – based detection inFluorescence Correlation Spectroscopyand measurements in living zebrafish embryosDissertation zur Erlangung des akademischen GradesDoctor rerum naturalium (Dr. rer. nat.)vorgelegt vonMarkus Burkhardtgeboren in Dresden am 19.09.1978Mai 2010First Referee: Prof. Dr. Petra SchwilleSecond Referee: Prof. Dr. Thorsten WohlandSubmitted on: 31 May 2010Oral examination: 7 September 2010AbstractFluorescence correlation spectroscopy (FCS) is an ultra sensitive optical technique to investigate thedynamic properties of ensembles of single fluorescent molecules in solution. It is in particular suitedfor measurements in biological samples. High sensitivity is obtained by employing confocal mi croscopy setups with diraction limited small detection volumes, and by using single photon sensitivedetectors, for example avalanche photo diodes (APD). However, fluorescence signal is hence typicallycollected from a single focus position in the sample only, and several measurements at dierent posi tions have to be performed successively.To overcome the time consuming successive FCS measurements, we introduce electron multi plying CCD (EMCCD) camera based spatially resolved detection for FCS. With this new detectionmethod, multiplexed FCS measurements become feasible.