Einsatz von Fret-Flim zur Beobachtung von Protein-Protein_Wechselwirkungen in lebenden Zellen: Einblicke in Interaktionen des präsynaptischen Proteins Bassoon [Elektronische Ressource] = Investigating protein-protein interactions by fret-flim in living cells: mediated by the presynaptic proteins bassoon / von Mini Jose

EINSATZ VON FRET-FLIM ZUR BEOBACHTUNG VON PROTEIN-PROTEIN WECHSELWIRKUNGEN IN LEBENDEN ZELLEN: EINBLICKE IN INTERAKTIONEN DES PRÄSYNAPTISCHEN PROTEINS BASSOON INVESTIGATING PROTEIN-PROTEIN INTERACTIONS BY FRET-FLIM IN LIVING CELLS: INSIGHTS INTO INTERACTIONS MEDIATED BY THE PRESYNAPTIC PROTEIN BASSOON Dissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) genehmigt durch die Fakultät für Naturwissenschaften der Otto-von-Guericke-Universität Magdeburg von Master of Science in Physics Mini Jose geb. am 26.12.1979 in Kanjirapally, Kerala, India Gutachter: Prof. Dr. Eckart D. Gundelfinger Prof. Dr. Fred S. Wouters eingereicht am 24. April 2007 verteidigt am 25. October 2007 Dedicated to my loving father, late Prof. Dr. P.K. Jose, Director, School of Distance Education, Mahatma Gandhi University, Kerala, India without whose support, I would never have achieved what I have. ACKNOWLEDGEMENTS First of all, I thank my dear friend, husband and colleague Deepak who has always been there to support me during the hardest of times and for all his help in preparing this work. I thank my dearest daughter Naina who has tolerated all the hardships with me during the last months of work. I sincerely thank Dr.