Einblicke in die molekularen Mechanismen zur Regulierung der Aktivität von Multidomain-Proteinen in lebenden Zellen mit Hilfe von FRET-FLIM-Untersuchungen [Elektronische Ressource] = Insights into molecular mechanisms regulating the activity of multidomain proteins in living cells using FRET-FLIM / von Deepak Kumaran Nair

EINBLICKE IN DIE MOLEKULAREN MECHANISMEN ZUR REGULIERUNG DER AKTIVITÄT VON MULTIDOMAIN-PROTEINEN IN LEBENDEN ZELLEN MIT HILFE VON FRET-FLIM UNTERSUCHUNGEN INSIGHTS INTO MOLECULAR MECHANISMS REGULATING THE ACTIVITY OF MULTIDOMAIN PROTEINS IN LIVING CELLS USING FRET-FLIM Dissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) genehmigt durch die Fakultät für Naturwissenschaften der Otto-von-Guericke-Universität Magdeburg Von Master of Science in Physics Deepak Kumaran Nair geb. am 10.03.1980 in Mararikulam, Kerala, India Gutachter: Prof. Dr. Stephan Diekmann Privatdozent Dr. Reinhard König eingereicht am 01. Oktober 2007 verteidigt 19. März 2008 Dedicated to my family and teachers for their love and support, which have encouraged and motivated me to achieve what I have… ii ACKNOWLEDGEMENTS I thank my wife Mini, whose love and support has inspired me in research and life. I thank my daughter Naina, who along with my wife has silently suffered all the hardships in the last few months. I sincerely thank Prof. Eckart Gundelfinger for motivating me to learn and appreciate cell biology and I will always be grateful for his help and consideration shown towards the successful completion of my work. I thank Dr. Werner Zuschratter and Dr.