Effects of metal-induced oxidative stress on endothelial cells in vitro [Elektronische Ressource] = Auswirkung von Metall-induziertem oxidativen Stress auf Endothelzellen in vitro / Roman Tsaryk

Effects of metal-induced oxidative stress on endothelial cells in vitro Auswirkung von Metall induziertem oxidativen Stress auf Endothelzellen in vitro Dissertation Zur Erlangung des Grades Doktor der Naturwissenschaften Am Fachbereich Biologie der Johannes Gutenberg-Universität Mainz Roman Tsaryk geb. am 09.06.1981 in Lviv Mainz, 2009ii Tag der mündliche Prüfung:12.03.2010 iii Contents 1. Introduction ......................................................................................................................................... 1 1.1. Metallic materials, implant integration and aseptic loosening of implants .......................... 1 1.1.1. Metallic materials for implantation ................................................................................. 1 1.1.2. Integration of metal implants and the role of endothelial cells ...... 2 1.1.3. Aseptic loosening and metal corrosion ........... 5 1.2. Oxidative stress ................................................................................................................... 8 1.2.1. ROS formation and antioxidant defence mechanisms .......................... 8 1.2.2. Oxidative DNA damage and repair ...................... 11 1.2.3. p53 pathway and cell cycle regulation ................................................................................ 13 1.3. HIF-1 pathway......................................................... 17 1.4. Aim of the study ..