Effect of Se-fertilization on the growth and S-metabolism of broccoli (Brassica oleracea var. italica) [Elektronische Ressource] / presented by Fu-Chen Hsu

DISSERTATION submitted to the Combined Faculties for the Natural Sciences and for Mathematics Of the Ruperto-Carola University of Heidelberg, Germany For the degree of Doctor of Natural Sciences presented by Master of Science Fu-Chen Hsu born in: Changhua, Taiwan Oral examination: Effect of Se-fertilization on the growth and S-metabolism of Broccoli (Brassica oleracea var. italica) Referees: Professor Dr. Thomas Rausch Professor Dr. Rüdiger Hell Acknowledgments This work has been carried out in Thomas Rausch’s laboratory at HIP Heidelberg, Germany. I am very grateful to Prof. Thomas Rausch for giving me the opportunity to pursue my PhD under his supervision. I would also like to express my gratitude to the members of my thesis advisory committee, Prof. Rüdiger Hell, Prof. Michael Wink, and Prof. Luise Krauth-Siegel for their advice. I am deeply indebted to Dr. Markus Wirtz and Muhammad Sayyar Khan for their work on S-metabolites HPLC experiments and to Dr. Ute Krämer for her help on the elemental analysis. I am very obliged to Dr. Jochen Bogs and Simon Heppel for their help on the luciferase assay and to Dr. Andreas Wachter for his teaching on the real-time PCR analysis. I would like to thank Britta Kupfer for her help on the total glucosinolate quantification and translating the summary in this thesis into German.