Effect of nitric oxide and inflammatory mediators on axonal transport [Elektronische Ressource] / submitted by Massimiliano Stagi

Effect of nitric oxide and inflammatory mediators on axonal transport PhD Thesis In partial fulfillment of the requirements for the degree “ Dr. rer. nat” AT THE GEORG-AUGUST UNIVERSITY GÖTTINGEN Submitted by Massimiliano Stagi Born in Montevarchi, Italy 2005 Declaration This thesis has been written independently and with no other sources and aids than stated. Massimiliano Stagi Date __________________________ Signature _____________________ 2 Chapter 1 : ABSTRACT ……………………………………………………………………… p. 5 Chapter 2 : INTRODUCTION ……………………………………………………………….. p.7 2.1 Molecular mechanisms of brain injury………………………. p.7 2.2 Microglial activation in the central nervous system…………………………………………. p.7 2.3 Motor proteins and axonal transport……………....………………………………. p.9 2.4 Mitochondria in the axon……………….……………………………………………………. p.10 2.5 Adaptor proteins for kinesin…………………………………………………………………. p.11 2.6 Technical approach to determine dynamics of axonal cargos ……………………. p.11 2.7 Technical approach to determine interaction between motors and tubuline track…..…p.12 Chapter 3: MATERIAL AND METHODS…………………………………………………….p.14 3.1 Neuronal cultures and transfection with EGFP or synaptophysin-EGFP……………………. p.14 3.2 Microglial cultures…………………………………………………………...………………. p.15 3.3 Transfection of neurons with pMH4.mito-YFP…………..